反应详情

113 mg (0.35 mmol) TBTU, 84 μL (0.60 mmol) triethylamine and 39 mg (0.30 mmol) 2-morpholin-4-yl-ethanol were added to a solution of 230 mg (0.29 mmol) (R)-1-(4-amino-3-chloro-5-trifluoromethyl-benzyl)-2-(1′-carboxymethyl-4,4′-bipiperidinyl-1-yl)-2-oxo-ethyl 4-(7-methoxy-2-oxo-1,2,4,5-tetrahydro-1,3-benzodiazepin-3-yl)-piperidine-1-carboxylate in 5 mL DMF and the reaction mixture was stirred overnight at RT. The reaction solution was poured onto a saturated NaHCO3 solution, the product precipitated was suction filtered and dried at 40° C. The crude product was dissolved in 25 mL dry isopropanol and precipitated with 0.5 M HCl in isopropanol as the salt. The precipitate was filtered off, washed with 5 mL isopropanol and 30 mL DIPE and dried overnight in the vacuum drying cupboard at 30° C.

1. customthe product precipitated
2. filtrationfiltered
3. customdried at 40° C
4. dissolutionThe crude product was dissolved in 25 mL dry isopropanol
5. customprecipitated with 0.5 M HCl in isopropanol as the salt
6. filtrationThe precipitate was filtered off
7. washwashed with 5 mL isopropanol and 30 mL DIPE
8. customdried overnight in the vacuum
9. customdrying cupboard at 30° C.