Water → HCID 68849560, compound

Participants

Reaction Conditions

Reaction Steps

40 mg of the ethyl 1-{9-[(4S,3R,5R)-3,4-dihydroxy-5-(hydroxymethyl)oxolan-2-yl]-6-(cyclopentylamino)purin-2-yl}pyrazole-4-carboxylate prepared in Example 3 was dissolved in 2 mL of 1 N KOH in methanol. 200 mL of water was then added and the mixture stirred at at 50° C. for 24 hours. The solvent was then removed and the residue dissolved in 3 mL water and brought to a pH of 3-4 by addition of 37% HCl. The resulting solid was collected by filtration and washed with water and ether then air dried to provide the product, 1-{9-[(4S,3R,5R)-3,4-dihydroxy-5-(hydroxymethyl)oxolan-2-yl]-6-(cyclopentylamino)purin-2-yl}pyrazole-4-carboxylic acid.

Provenance & Evidence