Trifluoroacetic acid → HCID 131727396, compound

Participants

Reaction Steps

To a cold (0° C.) solution of tert-butyl 4-{[2-(4-fluorobenzyl)-8,9-dihydroxy-1,6-dioxo-1,3,4,6-tetrahydro-2H-pyrido[1,2-a]pyrazin-7-yl]methyl}piperidine-1-carboxylate (1.12 g, 2.23 mmol) in methylene chloride (10 mL), trifluoroacetic acid (3.4 mL, 45 mmol) was added and the mixture was stirred for 2 hours. The mixture was concentrated in vacuo and the residue was purified by prep HPLC (Waters prep LC 4000 System using a Waters Nova Pak column [3*10×40 mm I.D. cartridges, C18, 6 μM pore size) eluting with 95-5% water (0.10% TFA)/acetonitrile (0.10% TFA) gradient at 60 mL/min]. Combined and concentrated the product fractions to give a yellow solid. The solids were triturated with acetonitrile and collected by vacuum filtration.

Provenance & Evidence