Pyridine → HCID 54715410, compound

Participants

Reaction Steps

To a solution of 2-(4-fluorobenzyl)-8,9-dihydroxy-7-(piperidin-4-ylmethyl)-3,4-dihydro-2H-pyrido[1,2-a]pyrazine-1,6-dione mono TFA salt [50 mg, 0.125 mmol; Example 31 (Step 3)] in methylene chloride (2 mL), pyridine (12 μL, 0.149 mmol), trifluoroacetic anhydride (26 mg, 0.149 mmol) and a couple drops of DMF were added. After one hour, the reaction mixture was concentrated under vacuum, and the residue was purified by prep HPLC [Gilson semi preparative HPLC system using a Waters Nova Pak column (10×40 mm I.D. cartridge, C18, 6 μM pore size) eluting with 95-5% water (0.10% TFA)/acetonitrile (0.10% TFA) gradient at 35 mL/min]. The product fractions were collected and lyophilized to provide the title compound as a white solid.

Provenance & Evidence