Phosphate → 2-(2-methyl-phenyl)-N-methyl-2-hydroxy-acetamide

Beteiligte Stoffe

Reaktionsschritte

Using the plasmid pTrcGSRxc, E. coli HB101 was transformed. The resulting transformant was inoculated in a sterilized LB medium (100 ml) containing 0.1 mM of IPTG and 50 μg/ml of ampicillin, and was shaking-cultured (37° C., 15 hours). The resulting culture was centrifuged to obtain 0.1 g of wet bacterial cells. To a reaction tube were added 1.5 mg of 2-(2-methyl-phenyl)-N-methyl-2-oxo-acetamide, 0.1 g of the wet bacterial cells, 10 mg of NADPH, and 1.5 ml of a 100 mM phosphate buffer solution (pH 7), and these were reacted at a stirring rate of 1000 rpm at 30° C. for 20 hours. After the completion of the reaction, 5 ml of ethyl acetate was added to the reaction solution, and this was centrifuged (1000×g, 5 minutes) to recover an organic layer. The organic layer was distilled off to obtain oily 2-(2-methyl-phenyl)-N-methyl-2-hydroxy-acetamide. Content analysis by liquid chromatography under the following condition showed that the conversion rate was 87.2%. 1H-NMR analysis result of the resulting 2-(2-methyl-phenyl)-N-methyl-2-hydroxy-acetamide is shown below.

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