反应详情

Crude tert-butyl 4-(2-(dimethylamino)ethyl)-4-(3-(dimethylcarbamoyloxy)phenylcarbamoyl)piperidine-1-carboxylate from step C was treated with 1:1 TFA:CH2Cl2 (1.5 mL) for 45 min. The reaction was concentrated under vacuum, and the residue was purified by prep HPLC (30×100 mm C18 column, 0-50% MeOH:H2O (0.1% TFA), 12 min., 45 mL/min.) and lyophized to give impure title compound (173 mg) as the TFA salt. MS (ES+) [M+H]+=363.

1. concentrationThe reaction was concentrated under vacuum
2. customthe residue was purified by prep HPLC (30×100 mm C18 column, 0-50% MeOH:H2O (0.1% TFA), 12 min., 45 mL/min.)