1,2-Dimethoxyethane → 3-{6-[2-(2,4-Dichloro-phenyl)-ethylamino]-2-methoxy-pyrimidin-4-yl}-benzoic acid ethyl ester

Participants

Reaction Conditions

Reaction Steps

Cs2CO3 solution (407 mg, 1.25 mmol in 2 mL water) is added to a stirred solution of (6-chloro-2-methoxy-pyrimidin-4-yl)-[2-(2,4-dichloro-phenyl)-ethyl]-amine (166 mg, 0.5 mmol) and ethylcarbonylphenyl boronic acid (135.8 mg, 0.7 mmol) in 1,2-dimethoxyethane (5 mL). The mixture is degassed over nitrogen for 10 minutes, tetrakis(triphenylphosphine)palladium (0) (23 mg, 0.02 mmol) is added and the reaction mixture is refluxed at 90° C. for 6 hours. The reaction is cooled to room temperature, diluted with water (10 mL), filtered over a pad of Celite and the volatiles are removed under reduced pressure. The aqueous pH is adjusted to neutral (0.1 N HCl) and extracted twice with ethyl acetate. The combined extracts are washed with brine and water, dried over magnesium sulfate, filtered and concentrated in reduced pressure. The crude residue is purified by chromatography (SiO2 packed column), eluting with 5-15% Ethyl acetate/DCM to afford 3-{6-[2-(2,4-dichloro-phenyl)-ethylamino]-2-methoxy-pyrimidin-4-yl}-benzoic acid ethyl ester (48 mg, Example 77). LCMS: RT=2.95 minutes, MS: 447 (M+H); 1H NMR, (300 MHz, CDCl3): □8.65 (1H, s), 8.3 (1H, d, J=3.5 Hz), 8.15 (1H, d, J=3.5 Hz), 7.55 (1H, t, J=3.5 Hz), 7.45 (1H, s), 7.25 (2H, s), 6.5 (1H, s), 4.95 (1H, b), 4.45 (2H, q, J=3.5 Hz), 4.05 (3H, s), 3.75 (2H, b), 3.1 (2H, t, J=3.5 Hz), 1.45 (3H, t, 3.5 Hz). IC50=149 nM

Provenance & Evidence