(2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanal → Kanamycin

Participants

Reaction Conditions

Reaction Steps

A simulated kanamycin culture broth is prepared by sterilizing 160 ml glucose-bouillon medium under heating at 120° C for 15 minutes and adding kanamycin sulfate with 60 mg activity thereto. Then, the solution is passed through a column, 2 cm in diameter, wherein 6 g (dry weight) selective adsorbents containing activated charcoal as obtained in Example 1, after being sufficiently swelled with water, are packed, at the rate of 0.5 ml/minute. The column is then washed and further eluted with 0.1N HCl-methanol (1 : 1). Active fractions are combined, wherefrom kanamycin with 54 mg activity (90% recovery) is obtained in high purity and high yield (decoloration degree: 99%; purity: 98%).

Provenance & Evidence