Sodium Chloride → Aldosterone

Participants

Reaction Steps

Male rats of the Sprague-Dawley strain weighing about 180 g were surrenalectomized and at that moment, the rats received with their drinking water physiological serum. On the 4th day, the animals were fasted for 16 hours and then received as drinking water water containing 5% glucose. The test product was administered orally at the end of 16 hours in the form of a suspension in 0.25% carboxymethylcellulose. One hour after the administration of the product, they received intraperitoneally a surcharge of aqueous sodium chloride at a rate of 5 ml per 100 g of body weight of 9% physiological serum and subcutaneously 1 μg/kg of an alcoholic solution of 2.5% of aldosterone acetate. The animals were placed in diuresis cages without food or drink for 4 hours. At the end of this time, a miction was forced by pressure on the bladder and the volume of urine was adjusted to 50 ml and the amount of sodium and potassium was determined with an autoanalyser. The results expressed as the percentage of inhibition of the activity of 1 μg/kg of aldosterone monoacetate injected subcutaneously with the ratio of ##EQU1## in the surrenalectomized rats is reported in the following Table.

Provenance & Evidence