Water → HCID 67032570, compound

Participants

Reaction Steps

The crude 3-{2-[4-(benzyloxy)-phenyl]morpholin-4-yl}-propionic acid tert-butyl ester (see §3) was treated with a mixture of TFA, water, and CH2Cl2 (30:3:67; 3 mL) and stirred at RT for 2 h. Subsequently, the volatiles were removed in vacuo, and the crude product was purified by preparative HPLC to afford 3-{2-[4-(benzyloxy)-phenyl]-morpholin-4-yl}-propionic acid trifluoroacetic acid salt; Rt=1.86 min. (System A), [M+H]+ Found: 342.19; Calc: 342.17. Conditions for the preparative LC-MS: Injection of the crude product dissolved in 600 L DMSO/CH3CN 1:2; column Waters Sunfire 19×100 mm 5 μm 45° C., mobile phase water/CH3CN/TFA 0.1% 25 mL/min, run 20 min 10%-90% CH3CN, detection with UV 210-260 nm.

Provenance & Evidence