反应详情

To a solution of 155 mg of methyl 3-[[2-(2,6-bis-hydroxymethyl-pyridin-4-yloxy)-ethyl]-(2-nitro-benzenesulfonyl)-amino]-propanoate in 6 ml of acetonitrile, under argon, was added 325 mg of caesium carbonate and 67 μl of thiophenol. The mixture was stirred for 4 hours at room temperature and then filtered through a sinter of porosity 4. The cake was washed with EtOAc and the filtrate was concentrated under reduced pressure and purified on a Mega BE-SCX, 2GM 12ML cartridge (Varian), using washing with MeOH and detachment of the expected product with a 2N solution of ammonia in MeOH. The fractions containing the desired product were combined and concentrated under reduced pressure. 82 mg of methyl 3-[2-(2,6-bis-hydroxymethyl-pyridin-4-yloxy)-ethylamino]-propanoate were obtained. LC/MS (B): tr=0.33 min; [M+H]+: m/z 285.

1. filtrationfiltered through a sinter of porosity 4
2. washThe cake was washed with EtOAc
3. concentrationthe filtrate was concentrated under reduced pressure
4. custompurified on a Mega BE-SCX, 2GM 12ML cartridge (Varian)
5. washusing washing with MeOH and detachment of the expected product with a 2N solution of ammonia in MeOH
6. additionThe fractions containing the desired product
7. concentrationconcentrated under reduced pressure