2,4,6-Trimethylpyridine → HCID 67981371, compound

Participants

Reaction Steps

N-(2-Aminoethyl)maleimide TFA salt (51 mg, 0.20 mmol) and Eei-AOAc-OSu (77 mg, 0.30 mmol) were dissolved in NMP (2 mL). Sym.-collidine (80 μL, 0.6 mmol) was added and the reaction mixture stiffed for 70 min. The reaction mixture was diluted with water (7 mL) and the product, eei-aminooxyacetyl-maleimide, purified by semi-preparative HPLC. Purification using semi-preparative HPLC (gradient: 15-30% B over 40 min where A=water/0.1% acetic acid and B=ACN) affording 43 mg (75%) pure Eei-aminooxyacetyl-maleimide. The purified material, eei-aminooxyacetyl-maleimide, was characterised by LC-MS (gradient: 10-40% B over 5): tR: 1.93 min. found m/z: 284.1, expected MH+: 284.1

Provenance & Evidence