Methylamine → HCID 66548857, compound

Participants

Reaction Conditions

Reaction Steps

To a stirred solution of 3-hydroxy-3-{4-[5-(3,4,5-trichloro-phenyl)-5-trifluoromethyl-4,5-dihydro-isoxazol-3-yl]-phenyl}-cyclobutanecarboxylic acid (Preparation 4, 0.060 g, 0.118 mmol) in dry DMF (1 mL) was added HATU (0.067 g, 0.177 mmol), HOBt (0.0175 g, 0.129 mmol), DIPEA (0.03 g, 0.236 mmol) and saturated solution of methyl amine in THF (2 mL) at 0° C. Resulting reaction mixture was stirred 0° C. for 2 hours and at room temperature for 18 hours. After complete consumption of starting material, reaction mixture was quenched with water (5 mL), extracted with EtOAc (10 mL). Organic layer was washed with saturated solution of lithium chloride (2×20 mL), water (2×20 mL), dried over sodium sulphate and evaporated in vacuo to get brown crude. Crude was purified by column chromatography on silica gel (100-200 mesh size). Compound was eluted using 1.2% methanol in DCM to afford sticky mass, which was crystallized by chloroform:pentane (1:9) to afford white solid (0.018 g, 29%). 1H-NMR (400 MHz, CDCl3) δ: 2.49-2.52 (m, 2H), 2.81-2.83 (m, 2H), 2.84-2.86 (m, 1H), 2.88 (d, 3H, J=4.88 Hz), 3.67 (d, 1H, J=17.08 Hz), 4.07 (d, 1H, J=17.16 Hz), 5.67 (bs, 1H), 5.81 (s, 1H), 7.55 (d, 2H, J=8.4 Hz), 7.62-7.64 (m, 4H). LC-MS (m/z): [M−H] 518.80. HPLC purity: 98.48%.

Provenance & Evidence