Lithium Hydroxide → HCID 86758118, compound

Participants

Reaction Steps

To a solution of methyl 2-{(2E)-3-(3-amidinophenyl)-2-fluoro-N-[4-(2-sulfamoylphenyl)phenyl]but-2-enoylamino}acetate (100 mg, 0.19 mmol) in 5 ml methanol was added a 0.5N lithium hydroxide solution (1 ml, 0.5 mmol). The reaction was stirred at room temperature for 4 hours then concentrated and purified on a 2×25 cm Vydac C18 HPLC column to give 2-{(2E)-3-(3-amidinophenyl)-2-fluoro-N-[4-(2-sulfamoylphenyl)phenyl]but-2-enoylamino}acetic acid (70 mg, 71%) as a fluffy white powder after lyophilization. ES-MS (M+H+): 511.1.

Provenance & Evidence