Sodium borohydride → HCID 131723272, compound

Participants

Reaction Conditions

Reaction Steps

0.15 g of sodium borohydride was added in one portion, at a temperature in the region of 20° C. and under an inert atmosphere, to a stirred solution of 1.6 g of methyl (3R,4R)-4-[3-oxo-3-(6-methoxyquinolin-4-yl)propyl]-1-[3-(thien-2-yl)prop-2-ynyl]-piperidine-3-carboxylate in 15 cm3 of methanol. The mixture was subsequently stirred for 2.5 hours at a temperature in the region of 25° C. 15 cm3 of distilled water were then added dropwise over approximately 10 minutes while maintaining at a temperature in the region of 15° C. The mixture was concentrated under reduced pressure (5 kPa) at a temperature in the region of 40° C. The residue obtained was taken up in 20 cm3 of distilled water. The mixture was extracted with a total of 100 cm3 of dichloromethane. The organic phases were combined, dried over sodium sulfate, and then evaporated under reduced pressure (5 kPa) at a temperature in the region of 40° C. The solid obtained was purified by chromatography under argon pressure (50 kPa) on a column of silica gel (particle size 20-45 μm; diameter 3 cm; height 35 cm), elution was carried out with a mixture of ethyl acetate and cyclohexane (8/2 by volume), and 15-cm3 fractions were collected. Fractions 38 to 59 were combined and then evaporated under reduced pressure (2 kPa) at a temperature in the region of 40° C. 1.3 g of methyl (3R,4R)-4-[3-(R,S)-hydroxy-3-(6-methoxyquinolin4-yl)propyl]-1-[3-(thien-2-yl)prop-2-ynyl]piperidine-3-carboxylate were thus obtained in the form of a foam.

Provenance & Evidence