Phosphate → HCID 12068238, compound

Participants

Reaction Conditions

Reaction Steps

As the present enzyme, each of the various commercial enzymes shown in Table 1 was added in an amount shown in Table 2 to 1 ml of 100 mM phosphate buffer (pH 7.0) and dissolved therein. 10 mg of racemic ethyl (1SR,5RS,6SR)-2-oxobicyclo[3.1.0]hexane-6-carboxylate was dissolved in 1 ml n-hexane, then added to the enzyme solution, and heated to 40° C. and the mixture was stirred. After the time shown as the reaction time in Table 2 elapsed, an aliquot of the reaction solution was removed and analyzed by HPLC [column: Sumichiral OA-5000, 4.6 mmφ×15 cm (Shumika Bunseki Center)], and the optical purity and reaction yield of the formed 2-oxobicyclo [3.1.0]hexane-6-carboxylic acid were determined. (−)-(1R,5S,6R)-2-oxobicyclo [3.1.0]hexane6-carboxylic acid was obtained in excess. The results are shown in Table 2.

Provenance & Evidence