Formaldehyde → 4-[3-(methylamino)-1H-indazol-5-yl]-6-{2-[(2-methylpropyl)oxy]phenyl}pyrimidin-2(1H)-one

HRID 2189155Notes

Reaction equation

HRID 2189155 reaction equation
[NH2:1][C:2]1[C:10]2[C:5](=[CH:6][CH:7]=[C:8]([C:11]3[CH:16]=[C:15]([C:17]4[CH:22]=[CH:21][CH:20]=[CH:19][C:18]=4[O:23][CH2:24][CH:25]([CH3:27])[CH3:26])[NH:14][C:13](=[O:28])[N:12]=3)[CH:9]=2)[NH:4][N:3]=1.C=O.[C:31]([BH3-])#N.[Na+]>C(O)(=O)C.CN(C)C=O>[CH3:31][NH:1][C:2]1[C:10]2[C:5](=[CH:6][CH:7]=[C:8]([C:11]3[CH:16]=[C:15]([C:17]4[CH:22]=[CH:21][CH:20]=[CH:19][C:18]=4[O:23][CH2:24][CH:25]([CH3:26])[CH3:27])[NH:14][C:13](=[O:28])[N:12]=3)[CH:9]=2)[NH:4][N:3]=1

Participants

6

Reactant 3

HCID 712FormaldehydeHCID 5003444Sodium cyanoborohydrideHCID 251213724-(3-amino-1H-indazol-5-yl)-6-(2-isobutoxyphenyl)pyrimidin-2(1H)-one

Product 1

HCID 251228274-[3-(methylamino)-1H-indazol-5-yl]-6-{2-[(2-methylpropyl)oxy]phenyl}pyrimidin-2(1H)-oneYield 25%

Reagents, catalysts & solvents 2

HCID 176Acetic AcidCatalystHCID 6228DimethylformamideSolvent

Procedure

To a 5 mL round bottom flask were added commercially available 4-(3-amino-1H-indazol-5-yl)-6-(2-isobutoxyphenyl)pyrimidin-2(1H)-one (100 mg, 0.26 mmol), formaldehyde (0.12 g of 37% H2O solution, 1.6 mmol), dimethylformamide (2 mL) and glacial acetic acid (1 drop). The reaction mixture was stirred at room temperature for 1 h, after which time sodium cyanoborohydride (1M THF solution (Aldrich), 2 mL, 2 mmol) was added. The reaction mixture was stirred at room temperature for an additional 1 h. Formation of product was confirmed by LC/MS and the product was purified by preparatory HPLC (reverse-phase, acetonitrile/water with 0.1% formic acid) to yield 25 mg (25%) of the title compound. 1H-NMR (400 MHz, d6-DMSO): δ 11.78 (s, 1H), 8.62 (s, 1H), 8.04 (d, 1H), 7.61 (d, 1H), 7.49 (t, 1H), 7.30 (d, 1H), 7.19 (d, 1H), 7.10 (m, 2H), 6.23 (q, 1H), 3.85 (d, 2H), 2.86 (d, 3H), 2.01 (m, 1H), 0.93 (d, 6H). MS (EI) for C22H23N5O2: 390 (MH+).

Workup

  1. stirringThe reaction mixture was stirred at room temperature for an additional 1 h
  2. customthe product was purified by preparatory HPLC (reverse-phase, acetonitrile/water with 0.1% formic acid)

Sources & evidence

ORD record
ord-2d4d37c3b96a4ed6baf94dfcf57cc041
Source dataset
uspto-grants-2012_12
Patent number
US08324231B2

Reaction notes

Loading…