HCID 131730094, compound → HCID 131730095, compound

Participants

Reaction Steps

Compound 56.3 (65 mg, 0.14 mmol) was dissolved in concentrated NH4OH (1.5 mL) and stirred for 24 h and then concentrated in vacuo. The residue was subjected to reverse phase HPLC eluting with 5-50% CH3CN-water gradient to give 56.4 (52 mg, 93% yield): white solid (bis-ammonium salt); 1H NMR (D2O, 300 MHz) 8.11 (s, 1H), 7.89 (s, 1H), 6.20 (m, 2H), 5.86 (app t, J=16.0 Hz, 1H), 4.84 (d, J=52.8 Hz, 1H), 4.16 (m, 2H); 31P NMR (D2O 121.4 MHz) 9.6; 19F NMR (D2O, 282 MHz) −195 (m, 1F); MS (ESI) m/z 346 [M+H]+.

Provenance & Evidence