Hydrochloric Acid → HCID 59570937, compound

Participants

Reaction Steps

To a solution of (5-{[(2R)-2-[3-cyclopropyl-4-(cyclopropylsulfonyl)phenyl]-3-(tetrahydro-2H-pyran-4-yl)propanoyl]amino}pyrazin-2-yl)methyl acetate (120 mg) in methanol (4 mL) was added 1 M aqueous sodium hydroxide solution (1 mL) at room temperature, followed by stirring at room temperature for 2 hours. 1 M Hydrochloric acid was added to adjust the pH to 3, followed by extraction with ethyl acetate. The organic layer was washed with saturated aqueous sodium bicarbonate and saturated brine, and dried over anhydrous magnesium sulfate. The crude product obtained by concentration was purified by silica gel column chromatography (wetting with hexane:ethyl acetate=1:1, chloroform:methanol=1:0-10:1) to obtain (2R)-2-[3-cyclopropyl-4-(cyclopropylsulfonyl)phenyl]-N-[5-(hydroxymethyl)pyrazin-2-yl]-3-(tetrahydro-2H-pyran-4-yl)propanamide (93 mg) as a white amorphous substance.

Provenance & Evidence