反应详情

44 mg of 9-(4-{4-[(5S)-5-(acetylamino-methyl)-2-oxo-oxazolidin-3-yl]-2-fluoro-phenyl}-piperazin-1-yl)-8-fluoro-3-methyl-6-oxo-2,3-dihydro-6H-1-oxa-3,3a-diaza-phenalene-5-carboxylic acid ethyl ester (0.32 mmol) were heated at 80° C. in 2 ml of a 1/1 conc. HCl and acetic acid mixture. The reaction was monitored by HPLC. The HCl/AcOH mixture was evaporated, the residue dissolved in a methanol/dichloromethane 1/1 mixture, treated with triethylamine and evaporated. The deacetylated residue was dissolved in a 1/1 mixture acetic acid and acetic anhydride, and the reaction monitored by HPLC. The solvents were evaporated and the residue was purified by preparative HPLC.

1. customThe HCl/AcOH mixture was evaporated
2. dissolutionthe residue dissolved in a methanol/dichloromethane 1/1 mixture
3. additiontreated with triethylamine
4. customevaporated
5. dissolutionThe deacetylated residue was dissolved in a 1/1 mixture acetic acid and acetic anhydride
6. customThe solvents were evaporated
7. customthe residue was purified by preparative HPLC