Ethanol → HCID 131712879, compound

Participants

Reaction Steps

A solution of p-nitrobenzyl 2-benzyloxycarbonylmethylthio-6-(1-hydroxy)ethyl-carbapen-2-em-3-carboxylate (7.1 mg, 0.014 mmol) in dioxane (0.84 ml) is treated with ethanol (0.07 ml), water (0.49 ml) containing sodium bicarbonate (1.4 mg, 0.017 mmol), and 10% palladium on charcoal (7.1 mg) and the mixture is hydrogenated at 40 psi for 30 minutes. The mixture is diluted to 3 ml with water and centrifuged to remove the catalyst which is washed with more water (2×1 ml). The combined aqueous solution is washed with ethylacetate (4×2 ml), filtered through a cotton plug, concentrated in vacuo to ca. 1 ml, and lyophilized to provide the title compound as a powder: UV (0.1 M pH 7 phosphate buffer) 300 nm; electrophoresis (0.05 M pH 7 phosphate buffer, 2 KV, 25 ma, 20 mins) gives a single bioactive zone migrating 3.5 cm toward the anode.

Provenance & Evidence