Sodium Bicarbonate → HCID 131712882, compound

Participants

Reaction Steps

A solution of crude p-nitrobenzyl 2-(p-nitrobenzyloxycarbonyl)methylthio-6-(1-hydroxy)ethyl-carbapen-2-em-3-carboxylate (8.7 mg) in dioxane (1.20 ml) is diluted with ethanol (0.10 ml) and water (0.70 ml) containing sodium bicarbonate (2.3 mg). The resulting solution is treated with 10% palladium on charcoal (15 mg) and hydrogenated at 40 psi for 60 minutes. The mixture is diluted with water to 5 ml and centrifuged to remove the catalyst which is washed with more water (2×1 ml). The combined aqueous solution is washed with ethyl acetate (4×3 ml), filtered through a cotton plug, concentrated in vacuo to ca. 1 ml, and lyophilized to provide the title compound as a solid: UV (pH 7 phosphate buffer) 301 nm; electrophoresis (0.05 M pH 7 phosphate buffer 2 KV, 20 ma, 20 mins) gives a biactive zone migrating 9.3 cm toward the anode (dicarboxylate).

Provenance & Evidence