反应详情

The title compound was prepared following procedure described for example 4, step 1, but starting from Intermediate 3 (113.14 mg; 0.50 mmol) and Intermediate 34 (114.32 mg; 0.50 mmol). The reaction mixture was filtered through a SPE NH2 column (2 g) and rinsed with ACN. The filtrate was passed through a SPE SCX column (2 g) and rinsed with ACN. After evaporation of the solvents, the crude product was purified by flash chromatography (c-hex/EtOAc: 9.5/0.5), affording the title compound as an off-white solid. LC/MS (Method B): 419.3 (M−H)−, 460.3 (M+ACN)+. HPLC (Method A) Rt 6.51 min (Purity: 99.8%).

1. customThe title compound was prepared following procedure
2. filtrationThe reaction mixture was filtered through a SPE NH2 column (2 g)
3. washrinsed with ACN
4. washrinsed with ACN
5. customAfter evaporation of the solvents
6. customthe crude product was purified by flash chromatography (c-hex/EtOAc: 9.5/0.5)