Hydrochloric Acid → HCID 66628232, compound

Participants

Reaction Steps

At RT, a solution of lithium hydroxide (2.3 mg, 95.9 μmol, 5 eq.) in water (125 μl) was added to a solution of 2-((7R)-6-cyano-7-(4-cyanophenyl)-5-methyl-4-[3-(trifluoromethyl)phenyl]-4,7-dihydro[1,2,4]triazolo[1,5-a]pyrimidin-2-ylamino)-2-oxoethyl acetate (10.0 mg, 19.2 μmol; Example 31) in THF (625 μl). After 1 h of stirring, conversion was found to be complete. 1 N hydrochloric acid (153 μl, 8 eq.) was added, and the reaction mixture was directly purified by preparative HPLC (Kromasil C18 column, 5 μm, 50×20 mm; mobile phase: acetonitrile-water-0.1% TFA). After lyophilization, the product was obtained as a solid (6.5 mg, 71% of theory).

Provenance & Evidence