Ammonia → HCID 72205348, compound

Participants

Reaction Steps

A mixture of {3-[5-(5-chloro-2-methyl-phenyl)-6-(4-chloro-phenyl)-1-isopropyl-4-oxo-1,4,5,6-tetrahydro-pyrrolo[3,4-b]pyrrol-2-yl]-4-methoxy-phenyl}-acetic acid (Example 60) (0.177 mmol), EDC.HCl (0.222 mmol), HOBt (0.131 mmol) and Et3N (0.621 mmol) in CH2Cl2 (1.3 mL) at rt was treated with an ammonia solution (0.4N/THF) (0.52 mmol). After stirring for 16 h, the reaction mixture was diluted with H2O and extracted with CH2Cl2 (2×). The combined organic layers were successively washed with H2O and brine, dried (Na2SO4), filtered and concentrated. The residue was purified using a RediSep® silica gel column to afford the title compound as a white solid. tR: 7.08 min (HPLC 2); ESI-MS: tR=1.16 min, [M+H]+ 562/564 (LC-MS 1); TLC: Rf=0.14 (EtOAc).

Provenance & Evidence