反应详情

To a stirred solution of methyl 2-methyl-3-[methyl(oxan-4-yl)amino]-5-(1-methylazetidin-3-yl)benzoate (296 mg, 0.89 mmol) in THF (10 ml) and MeOH (1 ml) was added 2M NaOH solution (4.5 ml, 8.9 mmol) and the reaction was stirred at 50° C. for 16 h after which time 4M NaOH solution (1 ml, 4.5 mmol) was added and the reaction was heated for a further 24 h. The reaction was cooled to room temperature and the product was neutralised with 1M HCl and evaporated to dryness followed by azeotrope with toluene to remove residual water. The crude acid was taken through to the next stage without any purification. The crude residue of 2-methyl-3-[methyl(oxan-4-yl)amino]-5-(1-methylazetidin-3-yl)benzoic acid was suspended in DMF (10 ml) and the solution was cooled using an ice bath followed by the addition of DIPEA (433 μl, 2.49 mmol) and HATU (567 mg, 1.49 mmol). The reaction was stirred at 0° C. for 5 min after which time 3-(aminomethyl)-4,6-dimethyl-1,2-dihydropyridin-2-one (89%, 221 mg, 1.29 mmol) was added and the reaction was warmed room temperature for 16 h. The reaction mixture was then poured onto distilled water (50 ml) and the aqueous phase was extracted with DCM (3×50 ml), washed with brine (50 ml) dried (Na2SO4), filtered and evaporated to give an oil. The product was then purified using preparative HPLC method (and evaporated to dryness followed by trituration with ether and filtration to afford the title compound as an off-white solid (51 mg, 10%). LC-MS 91%, m/z=453.2, 1H NMR (500 MHz, MeOD) δ 7.14 (d, J=1.7 Hz, 1H), 7.09 (d, J=1.7 Hz, 1H), 6.12 (s, 1H), 4.46 (s, 2H), 4.41 (t, J=9.2 Hz, 2H), 4.22 (t, J=9.3 Hz, 2H), 4.14 (dd, J=17.4, 8.5 Hz, 1H), 3.98-3.89 (m, 2H), 3.36 (td, J=11.3, 3.4 Hz, 2H), 3.06 (ddd, J=14.6, 9.8, 4.9 Hz, 1H), 2.97 (s, 3H), 2.64 (s, 3H), 2.39 (s, 3H), 2.26 (s, 3H), 2.24 (s, 3H), 1.73-1.62 (m, 4H).

1. additionwas added
2. temperaturethe reaction was heated for a further 24 h
3. customevaporated to dryness
4. customto remove residual water
5. customThe crude acid was taken through to the next stage without any purification
6. temperaturethe solution was cooled
7. additionwas added
8. temperaturethe reaction was warmed room temperature for 16 h
9. additionThe reaction mixture was then poured
10. distillationonto distilled water (50 ml)
11. extractionthe aqueous phase was extracted with DCM (3×50 ml)
12. washwashed with brine (50 ml)
13. dry with materialdried (Na2SO4)
14. filtrationfiltered
15. customevaporated
16. customto give an oil
17. customThe product was then purified
18. customevaporated to dryness
19. filtrationfollowed by trituration with ether and filtration