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Hydroxylamine → HCID 178419078、化合物
関与物質
| 役割 | 名称 | HCID | 仕込み量 |
|---|---|---|---|
| 反応物 | Hydroxylamine | HCID 787 | — |
| 反応物 | Sodium Chloride | HCID 5234 | — |
| 反応物 | N-Hexylimidodicarbonimidic diamide | HCID 20977 | — |
| 反応物 | 4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid | HCID 23831 | — |
| 反応物 | Bistris | HCID 81462 | — |
| 反応物 | (2-(Trimethylammonium)ethyl)methanethiosulfonate | HCID 107933 | — |
| 反応物 | D-1,4-Dithiothreitol | HCID 446094 | — |
| 生成物 | HCID 178419078 | HCID 178419078 | — |
反応手順
Reactions were assembled in a volume of 260 μl containing 50 mM Hepes buffer, 150 mM NaCl, pH 7.5 and as indicated 5 μM SrtADN in 50 mM BisTris, pH 7.5, 10 μM LPXTG peptide (DABCYL-QALPETGEE-EDANS), 10 μM TGXLP peptide (DABCYL-QATGELPEE-EDANS), 5 mM MTSET, 0.2 M NH2OH, 5 mM pHMB or 10 mM DTT. Incubations were carried out for 1 hour at 37° C. Samples were analyzed in a fluorimeter using 395 nm for excitation and 495 nm for recordings.
情報源と証拠
| DOI | 10.6084/m9.figshare.5104873.v1 |
|---|---|
| 特許番号 | US07101692B2 |
| 出典データセット | uspto-grants-2006_09 |
| ORD ID | ord-74f5c15cb2534009a185745b826cea0d |
| 出典メモ | uspto-grants-2006_09 |