L-Tryptophan → (2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanal

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Reaction Steps

Trp-1+glucose medium, a minimal medium that has been used for increased production of tryptophan in E. coli cells (Zeman et al. Folia Microbiol. 35:200-4, 1990), was prepared as follows. To 700 mL nanopure water the following reagents were added: 2 g (NH4)2SO4, 13.6 g KH2PO4, 0.2 g MgSO4*7H20, 0.01 g CaCl2*2H20 and 0.5 mg FeSO4*7H20. The pH was adjusted to 7.0, the volume was increased to 850 mL, and the medium was autoclaved. A 50% glucose solution was prepared separately, and sterile-filtered. Forty mL was added to the base medium (850 mL) for a 1 L final volume.

Provenance & Evidence