Hydrochloric Acid → HCID 69652395, compound

Participants

Reaction Steps

Add lipase (1.7 g, Amano AY30) to a slurry of piperidine-1,3,5-tricarboxylic acid 1-tert-butyl ester 3,5-diethyl ester (5.8 g, 17.6 mmol) in pH 7.2 phosphate buffer (58 mL). Stir 48 H, dilute with water then acidify to pH 2.5 with 1N HCl. Extract 3× with EtOAc. Wash with brine, dried over Na2SO4, filter and concentrate to dryness to give the title compound (5.0 g, 94%) as a mixture of cis isomers as a white solid. MS(IS) 300.2 (M−1); Anal. Calc'd for C14H23NO6.0.5CHCl3: C, 54.91; H, 7.56; N, 4.56. Found: C, 54.90; H, 7.59; N, 4.55.

Provenance & Evidence