Adenosine → HCID 136205272, compound

Participants

Reaction Steps

2,6-diamino-9-(2-O-pentyl-β-D-ribofuranosyl)purine (1.9 g) in 0.1 M sodium phosphate buffer (50 ml, pH 6.0) and DMSO (25 ml) was treated with adenosine deaminase (added in two aliquots—first aliquot 50 mg, second aliquot 80 mg) at 35° C. as per the procedure of Example 3 to yield 1.4 g of product. 1H NMR (DMSO-d6) δ 0.8 (t, 3, CH3), 1.16 (m, 4, 2×CH2), 1.4 (m, 2, CH2), 3.38, 3.6 (m, 4, OCH2, H-5′), 3.93 (s, 1, H-4′), 4.28 (m, 2, H-2′, H-3′), 5.17 (br, 2, 5′, 3′-OH), 5.8 (d, 1, H-1′), 6.53 (br s, 2, NH2), 8.0 (s, 1, H-8) and 10.68 (br, 1, NH)).

Provenance & Evidence