4-Nitrophenyl butyrate → Cholesterol

Participants

Reaction Steps

The assay substrate contained p-Nitrophenyl butyrate (Sigma Cat. No. N9876) MW 209.2. Density 1.2 and Taurocholic acid, sodium salt (Sigma Cat. No. T 4009) MW 537.7 The assay substrate was prepared by dissolving 43 mg Taurocholic acid (6 mM final concentration)+87 μl p-Nitrophenyl butyrate (40 μM final concentration)+50 μl 20% Triton X-100 (0.08% final concentration) in 10 ml of 100 mM MOPS 2 mM CaCl2 pH 7.3. The substrate was shaken to dissolve the ingredients and centrifuged to clarify.

Provenance & Evidence