反应详情

温度

100 °C

To a mixture of 2,2,2-trifluoro-N-(3-hydroxy-1-{4-[9-phenyl-3-(2-pyrimidinyl)[1,2,4]triazolo[3,4-f]-1,6-naphthyridin-8-yl]phenyl}cyclobutyl)acetamide (4-3) (10 mg, 0.016 mmol) in EtOH (1.0 mL) was added NaOH (2M in water, 1.0 mL, 2.00 mmol), and the mixture was heated under microwave irradiation at 100° C. for 1 hour. The mixture was diluted with water, extracted with CHCl3, washed with brine, dried (MgSO4), filtered, and concentrated. The residue was purified by preparative TLC, eluting with 10% MeOH in CHCl3 to give 3-amino-3-{-4-[9-phenyl-2-(2-pyrimidinyl)[1,2,4]triazolo[5,1-f]-1,6-naphthyridin-8-yl]phenyl}cyclobutanol (6-1) as a colorless solid. MS (M+H)+: observed=486, calculated=486.

1. extractionextracted with CHCl3
2. washwashed with brine
3. dry with materialdried (MgSO4)
4. filtrationfiltered
5. concentrationconcentrated
6. customThe residue was purified by preparative TLC
7. washeluting with 10% MeOH in CHCl3