Phosphoric Acid → D-2-Aminohexano-6-lactam

Participants

Reaction Steps

Then 100 mg of these lyophilized cells from each monoculture were added to a reaction mixture consisting of 10 mM HEPES-NaOH buffer (pH 7.7), toluene (2,5 v/v %), pyridoxal-5-phosphate (20 μM), and L-α-amino-ε-caprolactam (2.5 m %). Chemical blanks (with the assay mixture without the lyophilized cells) were used as a negative control. All reaction mixtures (including the blanks) were incubated for approximately 72 hours at 40° C. before the reaction was stopped by the addition of 9 volumes of 1 M H3PO4. After removal of the cells by filtration through a 0.45 μM filter, these reaction mixtures were analyzed by the HPLC method described above to determine the concentration of D- and L-α-amino-ε-caprolactam. Finally, monocultures that formed D-α-amino-ε-caprolactam from the L-α-amino-ε-caprolactam substrate were sent to the NCIMB for strain determination. Nb. no D-α-amino-ε-caprolactam could be determined in the chemical blanks.

Provenance & Evidence