Water → 5-[8-[(6-chloroquinolin-4-yl)methyl]-6-(cyclopropylmethyl)-3-methyl-5-oxo-6,8-dihydro-5H-imidazo[1,2-c]pyrazolo[4,3-e]pyrimidin-9-yl]-1-methyl-1H-pyrrole-3-carbonitrile

HRID 2222237Notes

Reaction equation

[NH2:1][C:2]1[C:3]2[C:4](=[N:13][N:14]([CH2:24][C:25]3[C:34]4[C:29](=[CH:30][CH:31]=[C:32]([Cl:35])[CH:33]=4)[N:28]=[CH:27][CH:26]=3)[C:15]=2[C:16]2[N:20]([CH3:21])[CH:19]=[C:18]([C:22]#[N:23])[CH:17]=2)[N:5]([CH2:9][CH:10]2[CH2:12][CH2:11]2)[C:6](=[O:8])[N:7]=1.[CH2:36]1[CH2:46]CN2C(=NCCC2)C[CH2:37]1.ClCC(=O)C.O>CN(C=O)C.C(OCC)(=O)C>[Cl:35][C:32]1[CH:33]=[C:34]2[C:29](=[CH:30][CH:31]=1)[N:28]=[CH:27][CH:26]=[C:25]2[CH2:24][N:14]1[C:15]([C:16]2[N:20]([CH3:21])[CH:19]=[C:18]([C:22]#[N:23])[CH:17]=2)=[C:3]2[C:2]3[N:7]([C:36]([CH3:46])=[CH:37][N:1]=3)[C:6](=[O:8])[N:5]([CH2:9][CH:10]3[CH2:12][CH2:11]3)[C:4]2=[N:13]1

Participants

Conditions

Temperature: 125 °C

Procedure

5-[4-amino-2-[(6-chloroquinolin-4-yl)methyl]-7-(cyclopropylmethyl)-6-oxo-6,7-dihydro-2H-pyrazolo[3,4-d]pyrimidin-3-yl]-1-methyl-1H-pyrrole-3-carbonitrile (75 mg, 0.15 mmol) was dissolved in 1.5 mL anhydrous DMF with 30 mg DBU. Chloroacetone (28 mg, 0.3 mmol, 2.0 eq.) was added and the resulting solution heated in the microwave for 90 minutes at 125° C. Two addition eq. chloroacetone were added, and the reaction returned to the microwave and heated an additional 90 minutes at 125° C. The reaction mixture was diluted with 50 mL. water and 50 mL ethyl acetate. The aqueous layer was washed 3×50 mL with ethyl acetate. The combined organic fractions were washed with 50 mL brine and dried over sodium sulfate. The crude product was purified by chromatography on silica (gradient: dichloromethane to 10% methanol in dichloromethane), followed by reverse phase HPLC using 40% aqueous acetonitrile, yielding 20 mg product. 1H NMR (300 MHz, CHLOROFORM-D) d ppm 0.55 (m, 4H) 1.48 (m, 1H) 2.27 (d, J=0.94 Hz, 3H) 3.42 (s, 3 H) 4.13 (m, 2H) 5.82 (m, 2H) 6.62 (dd, J=4.52, 1.88 Hz, 1H) 6.76 (d, J=4.33 Hz, 1H) 7.36 (d, J=1.51 Hz, 1H) 7.44 (d, J=1.13 Hz, 1H) 7.70 (dd, J=9.04, 2.26 Hz, 1H) 7.90 (d, J=2.26 Hz, 1H) 8.10 (m, 1H) 8.81 (d, J=4.52 Hz, 1H); ES M+H+=524.

Workup

additionwere added
temperatureheated an additional 90 minutes at 125° C
additionThe reaction mixture was diluted with 50 mL
washThe aqueous layer was washed 3×50 mL with ethyl acetate
washThe combined organic fractions were washed with 50 mL brine
dry with materialdried over sodium sulfate
customThe crude product was purified by chromatography on silica (gradient: dichloromethane to 10% methanol in dichloromethane)

Sources & evidence

ORD record: ord-d3bbd59f98b044f18f86ba0e19566214
Source dataset: uspto-grants-2008_05
DOI: 10.6084/m9.figshare.5104873.v1
Patent number: US07371757B2

Reaction notes

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