HuagongsheHuagongshe Chemistry Print Report · For research reference only
Succinic Acid → Alpha-Cyano-4-Hydroxycinnamic Acid
Participants
| Role | Name | HCID | Charge |
|---|---|---|---|
| Reactant | Succinic Acid | HCID 1110 | — |
| Reactant | L-Leucine | HCID 6106 | — |
| Reactant | L-Threonine | HCID 6288 | — |
| Reactant | L-Tryptophan | HCID 6305 | — |
| Reactant | Sodium Hydroxide | HCID 14798 | — |
| Reactant | (2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanal | HCID 107526 | — |
| Reactant | Monobasic potassium phosphate | HCID 516951 | — |
| Reactant | (2R,3S,4S,5R)-2,3,4,5,6-pentahydroxyhexanal | HCID 3037556 | — |
| Reactant | HCID 178419078 | HCID 178419078 | — |
| Product | Alpha-Cyano-4-Hydroxycinnamic Acid | HCID 2102 | — |
Reaction Steps
SC-H agar: 7.5 g/l yeast nitrogen base without amino acids, 11.3 g/l succinic acid, 6.8 g/l NaOH, 5.6 g/l casamino acids without vitamins, 0.1 g/l tryptophan, and 20 g/l agar (Bacto). Autoclaved for 20 min. at 121° C. After autoclaving, 55 ml of a 22% galactose solution and 1.8 ml of a 5% threonine solution were added per 450 ml agar. YNB-1 agar: 3.3 g/l KH2PO4, 16.7 g/l agar, pH adjusted to 7. Autoclaved for 20 min. at 121° C. After autoclaving, 25 ml of a 13.6% yeast nitrogen base without amino acids, 25 ml of a 40% glucose solution, 1.5 ml of a 1% L-leucine solution and 1.5 ml of a 1% histidine solution were added per 450 ml agar.
Provenance & Evidence
| DOI | 10.6084/m9.figshare.5104873.v1 |
|---|---|
| Patent number | US06413559B1 |
| Source dataset | uspto-grants-2002_07 |
| ORD ID | ord-825e88adbf2b4870a7c292cc0810c294 |
| Source note | uspto-grants-2002_07 |