HuagongsheHuagongshe Chemistry Print Report · For research reference only
Lactate → L-Leucine
Participants
| Role | Name | HCID | Charge |
|---|---|---|---|
| Reactant | Lactate | HCID 91435 | — |
| Reactant | Pyruvate | HCID 107735 | — |
| Reactant | Nadh | HCID 439153 | — |
| Reactant | Fructose-1,6-Diphosphate | HCID 445557 | — |
| Product | L-Leucine | HCID 6106 | — |
Reaction Steps
Lactate dehydrogenase was measured by monitoring the oxidation of NADH at 340 nm in a reaction mixture with the following composition: 50 mM Tris-acetate buffer (pH 6), 0.5 mM fructose-1,6-diphosphate, 25 mM pyruvate and 0.5 mM NADH.
Provenance & Evidence
| DOI | 10.6084/m9.figshare.5104873.v1 |
|---|---|
| Patent number | US06413765B1 |
| Source dataset | uspto-grants-2002_07 |
| ORD ID | ord-145dc1006f434973a1eb0417565921e9 |
| Source note | uspto-grants-2002_07 |