Glutathione → 2,4-Diaminopyrimidin-5-ol

HRID 2227151Notes

Reaction equation

HRID 2227151 reaction equation
[NH2:1][C@@H:2]([CH2:6][CH2:7][C:8]([NH:10][C@H:11](C(NCC(O)=O)=O)CS)=O)[C:3]([OH:5])=[O:4].[CH:21]1[N:26]=[C:25]([NH2:27])[N:24]=[C:23]([NH2:28])[C:22]=1[OH:29]>>[CH:21]1[N:26]=[C:25]([NH2:27])[N:24]=[C:23]([NH2:28])[C:22]=1[OH:29].[NH2:1][C@H:2]([C:3]([OH:5])=[O:4])[CH2:6][C:7]1[N:24]=[CH:11][NH:10][CH:8]=1

Participants

4

Reactant 2

HCID 124886GlutathioneHCID 131268512,4-Diaminopyrimidin-5-ol

Product 2

HCID 131268512,4-Diaminopyrimidin-5-olHCID 178419078HCID 178419078, compound

Procedure

W3, W8, and W10 were further subcloned into a modified pGEX4T-1 plasmid (Pharmacia Biotech, Uppsala, Sweden) (where a NdeI cloning site was inserted between the BamHI and EcoRI sites) as NdeI-NotI fragments to generate glutathione S-transferase (GST) tagged fusion proteins. A similar strategy was used to generate fusion proteins without the (Gly4Ser)31inker (i.e., W4 (p27-p16), W7 (p2712-178-p16 fusion CDKi W9 (p2725-93-p16)). The nucleic acid and amino acid sequences of the p2725-93-p16 fusion CDKi, W9, without the HA tag and six histidine residues are provided in SEQ ID NO: 23 and SEQ ID NO: 24, respectively.

Sources & evidence

ORD record
ord-ba6c176d40b84966a7c2f1ff237718fc
Source dataset
uspto-grants-2002_07
Patent number
US06413943B1

Reaction notes

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