Nitrate → Sodium Nitrate

Participants

Reaction Steps

The potential inhibitory effect of nitrate on perchlorate reduction was investigated by co-incubating the DM-17 isolate with perchlorate and various levels of nitrate. The initial experiment started with 10 mM sodium perchlorate and either 0, 0.1, 1.0, 10 or 100 mM sodium nitrate, resulting in nitrate to perchlorate ratios of 0×, 0.01×, 0.1×, 1.0× and 10×, respectively. A subsequent experiment started with 1.0 mM of sodium perchlorate and either 0, 0.1, 1.0, 10 or 100 mM sodium nitrate, resulting in nitrate to perchlorate ratios of 0×, 0.1×, 1.0×, 10×, and 100×, respectively. Both experiments were conducted with BMS medium containing 100 mM sodium acetate. Test units consisted of 40 mL borosilicate vials with teflon lined septa. The headspace of each vial was purged with nitrogen gas prior to the start of the experiments. Single measurements of perchlorate, absorbance and pH were measured on each vial at 0, 24, 48 and 72 h. All vials were distributed in an environmental chamber using a fully randomized design. The incubation temperature was maintained at 30° C.

Provenance & Evidence