Indomethacin → (3H)Ltd4

HRID 2231670Notes

Reaction equation

[CH3:1][CH2:2][CH2:3][CH2:4][CH2:5]/[CH:6]=[CH:7]\[CH2:8]/[CH:9]=[CH:10]\[CH:11]=[CH:12]\[CH:13]=[CH:14]\[C@@H:15]([S:24][CH2:25][CH:26]([NH2:34])[C:27]([NH:29][CH2:30][C:31]([OH:33])=[O:32])=[O:28])[C@@H:16]([OH:23])[CH2:17][CH2:18][CH2:19][C:20]([OH:22])=[O:21].CC1[N:44](C(C2C=CC(Cl)=CC=2)=O)[C:43]2C=CC(OC)=CC=2C=1CC(O)=O>>[CH3:1][CH2:2][CH2:3][CH2:4][CH2:5]/[CH:6]=[CH:7]\[CH2:8]/[CH:9]=[CH:10]\[CH:11]=[CH:12]\[CH:13]=[CH:14]\[C@@H:15]([S:24][CH2:25][CH:26]([NH2:34])[C:27]([NH:29][CH2:30][C:31]([OH:33])=[O:32])=[O:28])[C@@H:16]([OH:23])[CH2:17][CH2:18][CH2:19][C:20]([OH:22])=[O:21].[NH2:44][CH2:43][CH2:25][C:26]1[N:34]=[CH:30][NH:29][CH:27]=1

Participants

Reactant 2

HCID 3715Indomethacin

HCID 6435286(3H)Ltd4

Product 2

HCID 6435286(3H)Ltd4

HCID 178419017HCID 178419017, compound

Procedure

Antagonism for LTD4 was determined in isolated guinea-pig trachea prepared as spiral strip. Tracheal preparations were suspended under 1 g tension in 10 ml organ baths containing 5 μmol of indomethacin and they were incuated for 1 hr prior to use. Contractile responses to LTD4 (2×10-8 g/ml) were obtained after the maximal response to histamine (10-4M). Test compounds dissolved in 100% dimethyl sulfoxide were added to the organ baths (final concentration of 10-6 g/ml) 30 min prior to LTD4 addition, and then contractile responses to LTD4 were compared with those of control which was obtained from a paired trachea in the absence of test compounds. LTD4 -induced contractions were expressed as a percentage of the maximal response to histamine. The antagonism was determined as follows:

Workup

customprepared as spiral strip

Sources & evidence

ORD record: ord-b49487d4ab7b49ff9147bd07d7b1dfe5
Source dataset: uspto-grants-1988_11
DOI: 10.6084/m9.figshare.5104873.v1
Patent number: US04783462

Reaction notes

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