beta-Maltose → beta-Maltose

HRID 2232954Notes

Reaction equation

[CH2:1]([OH:34])[C@H:2]1[O:7][C@H:6]([O:8][C@H:9]2[C@H:14]([OH:15])[C@@H:13]([OH:16])[C@@H:12]([O:17][C@H:18]3[C@H:23]([OH:24])[C@@H:22]([OH:25])[C@@H:21]([OH:26])[O:20][C@@H:19]3[CH2:27][OH:28])[O:11][C@@H:10]2[CH2:29][OH:30])[C@H:5]([OH:31])[C@@H:4]([OH:32])[C@@H:3]1[OH:33].[CH2:35]([OH:57])[C@H:36]1[O:41][C@H:40]([O:42][C@H:43]2[C@H:48]([OH:49])[C@@H:47]([OH:50])[C@H:46]([OH:51])[O:45][C@@H:44]2[CH2:52][OH:53])[C@H:39]([OH:54])[C@@H:38]([OH:55])[C@@H:37]1[OH:56]>C([O-])(=O)C>[CH2:35]([OH:57])[C@H:36]1[O:41][C@H:40]([O:34][CH2:1][C@H:2]2[O:7][C@H:6]([O:8][C@H:9]3[C@H:14]([OH:15])[C@@H:13]([OH:16])[C@@H:12]([O:17][C@H:18]4[C@H:23]([OH:24])[C@@H:22]([OH:25])[CH:21]([OH:26])[O:20][C@@H:19]4[CH2:27][OH:28])[O:11][C@@H:10]3[CH2:29][OH:30])[C@H:5]([OH:31])[C@@H:4]([OH:32])[C@@H:3]2[OH:33])[C@H:39]([OH:54])[C@@H:38]([OH:55])[C@@H:37]1[OH:56].[CH2:35]([OH:57])[C@H:36]1[O:41][C@H:40]([O:42][C@H:43]2[C@H:48]([OH:49])[C@@H:47]([OH:50])[C@H:46]([OH:51])[O:45][C@@H:44]2[CH2:52][OH:53])[C@H:39]([OH:54])[C@@H:38]([OH:55])[C@@H:37]1[OH:56]

Participants

Conditions

Temperature: 100 °C

Procedure

The activities of these enzymes were assayed as follows: The α-isomaltosylglucosaccharide-forming enzyme of the present invention was assayed for enzymatic activity by dissolving maltotriose in 100 mM acetate buffer (pH 6.0) to give a concentration of 2% (w/v) for a substrate solution, adding a 0.5 ml of an enzyme solution to a 0.5 ml of the substrate solution, enzymatically reacting the mixture solution at 35° C. for 60 min, suspending the reaction mixture by heating at 100° C. for 10 min, and quantifying maltose, among the isomaltosyl maltose and maltose formed in the reaction mixture, by high-performance liquid chromatography (abbreviated as “HPLC” hereinafter). HPLC was carried out using “YMC PACK ODS-AQ303 column” commercialized by YMC Co., Ltd., Tokyo, Japan, at a column temperature of 40° C. and a flow rate of 0.5 ml/min of water; and using “RI-8012”, a differential refractometer commercialized by Tosoh Corporation, Tokyo, Japan. One unit activity of the α-isomaltosylglucosaccharide-forming enzyme is defined as the enzyme amount that forms one micromole of maltose per minute under the above enzymatic reaction conditions.

Workup

customto give a concentration of 2% (w/v) for a substrate solution
customat 35° C.
customfor 60 min

Sources & evidence

ORD record: ord-19117e9ce4a440ca8d0bbbb390fadd07
Source dataset: uspto-grants-2010_05
DOI: 10.6084/m9.figshare.5104873.v1
Patent number: US07709230B2

Reaction notes

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