反应详情

To an aqueous solution having a final solid concentration of 0.2% (w/v) of panose, α-isomaltosylmaltose, α-isomaltosyltriose, α-isomaltosyltetraose, or cyclotetrasaccharide, were added 100 units/g solid of an isomaltodextranase specimen, obtained by the method in Experiment 15, except for using 100 or 3,000 units of the specimen for the aqueous solution of cyclotetrasaccharide, allowed to react at 40° C. and pH 5.5 for 24 hours, and kept at 100° C. for 20 min to suspend the enzymatic reactions. The saccharide composition for each reaction mixture was determined on HPLC. The conditions used in HPLC were: Column, “MCIGEL CK04SS” comercialized by Mitsubishi Chemical Industries, Ltd., Tokyo, Japan; 80° C., inner column temperature; 0.5 ml/min, a flow rate of water as an eluent; and detection, “RI-8012”, a diffraction refractometer commercialized by Tosoh Corporation, Tokyo, Japan. The results are in Table 18.

1. additionα-isomaltosyltriose, α-isomaltosyltetraose, or cyclotetrasaccharide, were added 100 units/g solid of an isomaltodextranase specimen
2. customobtained by the method in Experiment 15
3. customto react at 40° C.
4. waitkept at 100° C. for 20 min
5. custom80° C., inner column temperature