Tris(hydroxymethyl)aminomethane → D-(-)-Fructose

Participants

Reaction Steps

The glucose isomerase activity of the immobilized samples was estimated at 70° C. using a batch assay technique using small particles (≈80 mesh). The assay was carried out in 125 ml. Erlenmeyer flasks in which the dry enzyme was hydrated in substrate (1 M glucose, 0.1 M Tris, 0.1 M maleic acid, 4.1 mM MgSO4.7H2O and 5 mmM NaHSO4 at pH 8.0) for 10 minutes at room temperature. The assay reaction was started by placing the flask in a 70° C. shaker water bath. At 15, 30, 45, 60 and 75 minutes, a 0.05 ml. sample of the reaction mixture was removed and added to 1.0 ml. of 0.1M HClO4 solution to stop the reaction. The amount of fructose formed in each sample was then estimated by the cysteine H2SO4 method. The activity was calculated through regression analysis of fructose formed with respect to time, where a unit of activity represents one μ mole of fructose formed per minute under conditions of the assay.

Provenance & Evidence