Sodium Hydroxide → Sodium Gluconate

Participants

Reaction Steps

10 l of a sterile aqueous solution which contains 1.5 kg of D-glucose.H2O, 10 g of dry corn steep nutrient, 10 g of ammonium dihydrogen phosphate, 10 g of potassium dihydrogen phosphate and 5 g of magnesium sulphate, and which has a pH value of 6.5, is inoculated, in a fermenter, with a shaken culture of Acetobacter suboxydans ATCC 621 at a temperature of 30° C., and the mixture is aerated, while stirring. A pH value of 5.5-6.0 is maintained by metering in sodium hydroxide solution. After about 40 hours, when all of the glucose has been converted to gluconate, the mixture is cooled and centrifuged. Sodium gluconate can be obtained in crystalline form in a yield of over 90% by evaporating the centrifuged solution.

Provenance & Evidence