Sodium Hydroxide → Calcium gluceptate

HRID 2237328Notes

Reaction equation

HRID 2237328 reaction equation
[OH-].[Na+].[CH2:3]([OH:17])[C@@H:4]([OH:16])[C@@H:5]([OH:15])[C@H:6]([OH:14])[C@@H:7]([OH:13])[CH:8]([OH:12])[C:9]([O-:11])=[O:10].[CH2:18]([OH:32])[C@@H:19]([OH:31])[C@@H:20]([OH:30])[C@H:21]([OH:29])[C@@H:22]([OH:28])[CH:23]([OH:27])[C:24]([O-:26])=[O:25].[Ca+2:33].C([O-])(=O)C>>[CH2:3]([OH:17])[C@@H:4]([OH:16])[C@@H:5]([OH:15])[C@H:6]([OH:14])[C@@H:7]([OH:13])[CH:8]([OH:12])[C:9]([O-:11])=[O:10].[CH2:18]([OH:32])[C@@H:19]([OH:31])[C@@H:20]([OH:30])[C@H:21]([OH:29])[C@@H:22]([OH:28])[CH:23]([OH:27])[C:24]([O-:26])=[O:25].[Ca+2:33]

Participants

3

Reactant 2

HCID 14798Sodium HydroxideHCID 131728119HCID 131728119, compound

Product 1

HCID 62859Calcium gluceptate

Conditions

Temperature
25 °C

Procedure

This description is for labeling the liposomes with “BMEDA” because the 186Re-“BMEDA” complex produced the higher labeling efficiency and stability. “BMEDA” was synthesized using a modification of a procedure described by Corbin et al. in “Preparation and properties of tripodal and linear tetradentate N,S-donor ligands and their complexes containing the MoO22+ core” Inorganica Chimica Acta. 1984; 90:41-51. First, a 0.17 M glucoheptonate-0.1 M acetate solution was prepared and the pH adjusted to 5.0 with 5 M NaOH. Next, the “BMEDA” solution was prepared by pipetting 4.5 mg of “BMEDA” (4.5 μl) to a new vial and adding 2.1 ml of the glucoheptonate-acetate solution. After flushing the “BMEDA”-glucoheptonate-acetate solution with N2 gas for 20 min, the vial was sealed. The solution was stirred at 25° C. for 40 min. Next, 30 mg of SnCl2.2H2O was dissolved by adding with 2 drops of concentrated HCl in a new vial and 2.0 ml of sterile water added.

Workup

  1. customproduced the higher labeling efficiency and stability
  2. custom“BMEDA” was synthesized
  3. customdescribed by Corbin et al. in “Preparation and properties of tripodal and linear tetradentate N,S-donor ligands and their complexes
  4. customNext, the “BMEDA” solution was prepared
  5. customAfter flushing the “BMEDA”-glucoheptonate-acetate solution with N2 gas for 20 min
  6. customthe vial was sealed
  7. dissolutionNext, 30 mg of SnCl2.2H2O was dissolved
  8. additionby adding with 2 drops of concentrated HCl in a new vial and 2.0 ml of sterile water
  9. additionadded

Sources & evidence

ORD record
ord-70e822ffe3e34ea9991386949f378ca2
Source dataset
uspto-grants-2010_05
Patent number
US07718160B2

Reaction notes

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