4-Nitrophenol → HCID 131716968, compound

Participants

Reaction Steps

4.6 g (1.0×10-2 mol) of MA-Gly-Phe-Leu-Gly-OH was esterified with p-nitrophenol (1.1×10-2 mol) in 55 ml of THF by means of dicyclohexylcarbodiimide (DCC) (1.1×10-2 mol). After 3 hours at 15° C. and 12 hours at 25° C., the dicyclohexylurea was filtered off and the remaining solution was evaporated under reduced pressure. The residue was solidified by adding dry ethyl ether and after recrystalisation from acetone:ether (3:1) gave MA-Gly-Phe-Leu-Gly-ONp in 76% yield which was analytically pure; m.p. 124°-8° C. Aminoacid analysis gave Gly:Leu:Phe of 2.0:1.0:0.99.

Provenance & Evidence