Ammonia → 9-(2',3'-Dihydroxycyclopentan-1'-yl)adenine

Participants

Reaction Steps

(±)-(1R*,2S*,3R*)-3-(6-Chloro-9H-purin-9-yl)-1,2-cyclopentanediol (4.00 g, 15.7 mmol) and ammonia (1, 100 ml) were stirred in a Parr bomb at 25° C. for 18 hours. Evaporation and neutralization with 1N NaOH (16 ml), followed by elution of a silica gel column with MeOH:CHCl3 /1:4 gave title compound as white crystals, recrystallized from 95% ethanol (3.54 g, 96%); mp 201°-202° C.; 1H-NMR (Me2SO-d6) δ8.19 and 8.12 (both s, 2, purine H-2, H-8), 7.18 (br s, 2, NH2), 4.98 (d, J=6.6, 1, 2-OH), 4.76-4.64 (m) overlapping 4.70 (d, J=3.4, 2, H-3 and 1-OH), 4.42-4.34 (m, 1, H-2), 4.04-3.97 (m, 1, H-1), 2.5-1.9 and 1.7-1.6 (both m, 4, two CH2).

Provenance & Evidence