Phosphoenolpyruvate → L-Glutamic Acid

HRID 2250970Notes

Reaction equation

C([O-])(=O)CC(CC([O-])=O)(C([O-])=O)O.C=C(OP(O)(O)=O)C(O)=O.[NH2:24][C@H:25]([C:31]([O-:33])=[O:32])[CH2:26][CH2:27][C:28]([O-:30])=[O:29]>>[NH2:24][C@H:25]([C:31]([OH:33])=[O:32])[CH2:26][CH2:27][C:28]([OH:30])=[O:29]

Participants

Reactant 3

HCID 1005Phosphoenolpyruvate

HCID 31348Citrate

HCID 5460544L-glutamate(2-)

Product 1

HCID 33032L-Glutamic Acid

Procedure

Subsequently, the citrate synthase gene, phosphoenolpyruvate carboxylase gene and glutamate dehydrogenase gene derived from Escherichia coli were introduced into the SC17sucA strain.

Workup

additionwere introduced into the SC17sucA strain

Sources & evidence

ORD record: ord-3472fafcd05046aeba6a828d392ff618
Source dataset: uspto-grants-2008_01
DOI: 10.6084/m9.figshare.5104873.v1
Patent number: US07319025B2

Reaction notes

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