HCID 23329199, compound → HCID 13344800, compound

Participants

Reaction Steps

A mixture of benzyl 6-ethyl-3-(2-benzyloxycarbonyl aminoethyl)thio-7-oxo-1-azabicyclo[3.2.0]hept-2-ene-2-carboxylate (10 mg) and 10% Pd/C (20 mg) in 1:1 dioxane-H2O (4 ml) is hydrogenated at 40 psi for 1 hr. The mixture is filtered to remove the catalyst which is washed with more H2O (4 ml). The combined filtrate and washings are concentrated in vacuo to 2 ml and loaded onto a column of 200-400 mesh Dowex-50×4 (Na form) ion-exchange resin. The column is eluted with deionized H2O. The appropriate fractions are pooled and lyophilized to provide 6-ethyl-3-(2-aminoethyl)thio-7-oxo-1-azabicyclo[3.2.0]hept-2-ene-2-carboxylic acid.

Provenance & Evidence