Sodium Bicarbonate → HCID 178508522, compound

Participants

Reaction Steps

After 600 mg of 7β-[(Z)-2-(2-amino-4-thiazolyl)-2-[[(R S)-(carboxy)(3,4-diacetoxyphenylthio)methoxy]imino]acetamido]-3-[[(4-carboxy-3-hydroxy-5-isothiazolyl)thio]methyl]-3-cephem-4-carboxylic acid obtained in Example 4 (10) was suspended in 20 ml of water, saturated sodium hydrogencarbonate aqueous solution was added to the suspension at room temperature to adjust pH to 8. After stirring at room temperature for 2.5 hours, the aqueous solution was adsorbed to DIAION HP20 followed by elution with water. The fractions containing the desired product were collected. After concentration, the concentrate was freeze dried to give 150 mg of trisodium 7β-[(Z)-2-(2-amino-4-thiazolyl)-2-[[(R S)-(carboxy)(3,4-dihydroxyphenylthio)methoxy]imino]acetamido]-3-[[(4-carboxy-3-hydroxy-5-isothiazolyl)thio]methyl]-3-cephem-4-carboxylate.

Provenance & Evidence