Trifluoroacetic acid → HCID 178508509, compound

Participants

Reaction Steps

After 0.22 g of the compound obtained in Example 6 (7) was added to 25 ml of trifluoroacetic acid, the mixture was stirred at room temperature for an hour. The reaction mixture was cooled to 5° C. and 6 ml of water was added thereto. After stirring at 5° C., the temperature was reverted to room temperature followed by stirring for 2 hours. The solvent was distilled off under reduced pressure and ether was added to the residue to make powders. To the powders was added 100 ml of water and then 0.20 g of sodium hydrogencarbonate. The mixture was adsorbed to DIAION HP20 followed by elution with water-methanol. The fractions containing the desired product were collected. After concentration, the concentrate was freeze dried to give 90 mg (52.9%) of trisodium 7β-[(Z)-2-(2-amino-4-thiazolyl)-2-[[(R S)-(carboxy)(3-pyridyl)methoxy]imino]acetamido]-3-[[(4-carboxy-3-hydroxy-5-isothiazolyl)thio]methyl]-3-cephem-4-carboxylate.

Provenance & Evidence